Recombinant Vimentin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86911A
Western blot detection of Vimentin in C6,CHO-K1,Hela cell lysates using Vimentin Rabbit mAb(1:1000 diluted).Predicted band size:54kDa.Observed band size:54kDa.
Immunohistochemistry of Vimentin in paraffin-embedded Human tonsil using Vimentin Rabbit mAb at dilution 1:100
Immunofluorescence of Vimentin (green) in Hela using Vimentin antibody at dilution 1:20, and DAPI(blue)
Product Name
Recombinant Vimentin Monoclonal Antibody
Catalog Number
RD86911A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Vimentin,also named as VIM,belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells,especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092),suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Immunogen Information
Immunogen
A synthetic peptide of human Vimentin
Gene ID
7431
Swissprot
P08670
Synonyms
CTRCT30Epididymis luminal protein 113FLJ36605HEL113VIMVIMEVimentin
Calculated MW
134kDa
Observed MW
175kDa
Applications
Reactivity
Human,Mouse,Rat,Hamster
Tested Applications
WB,IHC-P,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.