Recombinant Vinculin Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86967A
Western blot detection of Vinculin in K562,Rat Brain,C6,3T3,Hela cell lysates using Vinculin Rabbit mAb(1:1000 diluted).Predicted band size:124kDa.Observed band size:124kDa.
Immunofluorescence of Vinculin(green) in Hela cells using Vinculin Rabbit mAb at dilution 1:50, and DAPI(blue)
Product Name
Recombinant Vinculin Monoclonal Antibody
Catalog Number
RD86967A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It plays an important role in cell morphology and locomotion.
Immunogen Information
Immunogen
A synthetic peptide of human Vinculin
Gene ID
7414
Swissprot
P18206
Synonyms
CMD1WCMH15Epididymis luminal protein 114HEL114MetavinculinMVMVCLOTTHUMP00000019861OTTHUMP00000019862VCLVINCVINCVinculin
Calculated MW
39kDa
Observed MW
50kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000, IF 1:50-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas