For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87092A
Western blot detection of Vinculin in K562,C6,3T3,Hela cell lysates using Vinculin Rabbit mAb(1:1000 diluted).Predicted band size:124kDa.Observed band size:124kDa.
Immunohistochemistry of Vinculin in paraffin-embedded Human breast cancer tissue using Vinculin Rabbit mAb at dilution 1:50
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It plays an important role in cell morphology and locomotion.
Immunogen Information
Immunogen
Recombinant protein of human Vinculin
Gene ID
7414
Swissprot
P18206
Synonyms
CMD1WCMH15Epididymis luminal protein 114HEL114MetavinculinMVMVCLOTTHUMP00000019861OTTHUMP00000019862VCLVINCVINCVinculin
Calculated MW
66kDa
Observed MW
66kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-P
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:50-1:200
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.