Recombinant WTAP Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86904A
Western blot detection of SPCS2/SPC25 in K562,Hela cell lysates using SPCS2/SPC25 Rabbit mAb(1:1000 diluted).Predicted band size:44kDa.Observed band size:55kDa.
Immunohistochemistry of WTAP in paraffin-embedded Human tonsil using WTAP Rabbit mAb at dilution 1:20
Product Name
Recombinant WTAP Monoclonal Antibody
Catalog Number
RD86904A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
WTAP,also named as KIAA0105 and hFL(2)D,is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle.
Immunogen Information
Immunogen
Recombinant protein of human WTAP
Gene ID
9589
Swissprot
Q15007
Synonyms
DKFZp686F20131Female-lethal(2)D homologFL2DhFL(2)DKIAA0105MGC3925Mum2OTTHUMP00000017522OTTHUMP00000017523PNAS 132PNAS-132PNAS132Pre mRNA splicing regulator WTAPPre-mRNA-splicing regulator WTAPPutative pre mRNA splicing regulator female letha
Calculated MW
43/41 kDa
Observed MW
44/42kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC-P,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IHC 1:20-1:100 IP 1:20-1:100
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas