Recombinant XBP1 Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87095A
Western blot detection of XBP1 in HL-60,U2OS cell lysates using XBP1 Rabbit mAb(1:1000 diluted).Predicted band size:29kDa.Observed band size:29kDa.
Product Name
Recombinant XBP1 Monoclonal Antibody
Catalog Number
RD87095A
Clonality
Monoclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.
Immunogen Information
Immunogen
Recombinant protein of human XBP1
Gene ID
7494
Swissprot
P17861
Synonyms
Tax responsive element binding protein 5Tax-responsive element-binding protein 5TREB5X box binding protein 1X box binding protein 2X-box-binding protein 1XBP 1XBP-1XBP1XBP1XBP2
Calculated MW
45kDa
Observed MW
65kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
300 μg/mL
Storage Buffer
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas