RHOG Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89180A
Immunohistochemistry of paraffin-embedded human liver cancer using RHOG Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using RHOG Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human appendix using RHOG Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using RHOG Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
RHOG Polyclonal Antibody
Catalog Number
RD89180A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]
Immunogen Information
Immunogen
A synthetic peptide of human RHOG
Gene ID
391
Swissprot
P84095
Synonyms
ARHGRHOG
Calculated MW
28kDa/34kDa/37kDa/42kDa/45kDa/47kDa
Observed MW
48kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.