For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89205A
Western blot analysis of extracts of various cell lines using RNF126 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using RNF126 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using RNF126 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions.
Immunogen Information
Immunogen
A synthetic peptide of human RNF126
Gene ID
55658
Swissprot
Q9BV68
Synonyms
RNF126
Observed MW
64kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.