RPA2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using RPA2 Polyclonal Antibody at dilution of 1:1000. 293T cells were treated by UV at room tempeRature for 15-30 minutes.
Immunohistochemistry of paraffin-embedded Human kidney cancer using RPA2 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RPA2 Polyclonal Antibody
Product Name
RPA2 Polyclonal Antibody
Catalog Number
RD82590A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Replication protein A 32 kDa subunitis aproteinthat in humans is encoded by theRPA2gene. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.
Immunogen Information
Immunogen
Recombinant fusion protein of human RPA2 (NP_002937.1).
Gene ID
6118
Swissprot
P15927
Synonyms
REPA2RP-A p32RP-A p34RPA32RPA2RP-Ap32RP-Ap34
Calculated MW
29 kDa/30 kDa/38 kDa
Observed MW
32 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas