RPL14 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81374A
Western blot analysis of extracts of various cell lines using RPL14 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using RPL14 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of MCF-7 cells using RPL14 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using RPL14 Polyclonal Antibody
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
RPL14 Polyclonal Antibody
Catalog Number
RD81374A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human RPL14 (NP_003964.3).
Gene ID
9045
Swissprot
P50914
Synonyms
RPL14CAG-ISL-7CTG-B33L14RL14hRL14
Calculated MW
23 kDa
Observed MW
28 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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