RPL27 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD81196A
Western blot analysis of extracts of various cell lines using RPL27 Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of C6 cells using RPL27 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using RPL27 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RPL27 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RPL27 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Product Name
RPL27 Polyclonal Antibody
Catalog Number
RD81196A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L27E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Immunogen Information
Immunogen
Recombinant fusion protein of human RPL27 (NP_000979.1).
Gene ID
6155
Swissprot
P61353
Synonyms
RPL27L27
Calculated MW
15 kDa
Observed MW
16 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.