RPL30 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81568A
Western blot analysis of extracts of various cell lines using RPL30 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RPL30 Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Rat heart using RPL30 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat heart using RPL30 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using RPL30 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using RPL30 Polyclonal Antibody at dilution of 1:100 (40x lens).
Confocal immunofluorescence analysis of HeLa cells using RPL30 Polyclonal Antibody at dilution of  1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RPL30 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
RPL30 Polyclonal Antibody
Catalog Number
RD81568A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human RPL30 (NP_000980.1).
Gene ID
6156
Swissprot
P62888
Synonyms
RPL30L30
Calculated MW
12 kDa
Observed MW
13 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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