RPL32 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81178A
Western blot analysis of extracts of various cell lines using RPL32 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using RPL32 Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Rat brain using RPL32 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using RPL32 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using RPL32 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using RPL32 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spinal cord using RPL32 Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse spinal cord using RPL32 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using RPL32 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RPL32 Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using RPL32 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
RPL32 Polyclonal Antibody
Catalog Number
RD81178A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human RPL32 (NP_000985.1).
Gene ID
6161
Swissprot
P62910
Synonyms
RPL32L32PP9932
Calculated MW
15 kDa
Observed MW
16 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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