RPL8 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using RPL8 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using RPL8 Polyclonal Antibody at dilution of 1:1000.
Confocal immunofluorescence analysis of HeLa cells using RPL8 Polyclonal Antibody at dilution of  1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RPL8 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using RPL8 Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using RPL8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
RPL8 Polyclonal Antibody
Catalog Number
RD80216A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human RPL8 (NP_000964.1).
Gene ID
6132
Swissprot
P62917
Synonyms
RPL8L8
Calculated MW
28 kDa
Observed MW
28 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:4000 IF 1:100-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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