RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89253A
Western blot analysis of extracts of various cell lines using RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver using RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat stomach using RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
RPS6KA1/RPS6KA2/RPS6KA3 Polyclonal Antibody
Catalog Number
RD89253A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Immunogen Information
Immunogen
A synthetic peptide of human RPS6KA1/RPS6KA2/RPS6KA3
Gene ID
6195/ 6196/ 6197
Swissprot
Q15418/Q15349/P51812
Observed MW
25KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.