RRP8 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89330A
Western blot analysis of extracts of various cell lines using RRP8 Polyclonal Antibody at 1:1000 dilution.
Variation
Quantity
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Overview
Product Name
RRP8 Polyclonal Antibody
Catalog Number
RD89330A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Essential component of the eNoSC (energy-dependent nucleolar silencing complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+/NADP(+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2 by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
Immunogen Information
Immunogen
A synthetic peptide of human RRP8
Gene ID
23378
Swissprot
O43159
Synonyms
NMLKIAA0409
Calculated MW
211kDa
Observed MW
200kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas