S-tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86720A
Western blotting with anti-S-Tag monoclonal antibody at dilution of 1:1000.Lane 1: S tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Product Name
S-tag Monoclonal Antibody
Catalog Number
RD86720A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. This interactive complex (S‐tag/S‐protein) results in micromolar affinity – dependent upon pH, temperature, and ionic strength . The S‐tag itself enables colorimetric detection via western blot analysis or a rapidly detected assay dependent upon ribonucleolytic reconstitution and activity.
Immunogen Information
Immunogen
Synthetic peptide corresponding to S tag conjugated to keyhole limpet haemocyanin.
Synonyms
KETAAAKFERQHMDS tagS peptideS peptide epitope tag
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:2000-1:4000
Concentration
2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.