SEC31A Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using SEC31A Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using SEC31A Polyclonal Antibody at dilution of 1:1000.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
SEC31A Polyclonal Antibody
Catalog Number
RD268016A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human SEC31A (NP_055748.2).
Gene ID
22872
Swissprot
O94979
Synonyms
SEC31AABP125ABP130HSPC275HSPC334SEC31L1
Calculated MW
56 kDa/105-134 kDa
Observed MW
165 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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