For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89451A
Western blot analysis of extracts of various cell lines using SETD3 Polyclonal Antibody at 1:1000 dilution.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3 ug SETD3 Polyclonal Antibody.Western blot was performed from the immunoprecipitate using SETD3 Polyclonal Antibody at a dilution of 1:1000.
Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.
Immunogen Information
Immunogen
A synthetic peptide of human SETD3
Gene ID
84193
Swissprot
Q86TU7
Synonyms
SETD3C14orf154SET domain containing 3
Calculated MW
89kDa
Observed MW
90KDa
Applications
Reactivity
Human
Tested Applications
WB,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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