SFPQ Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD80169A
Western blot analysis of extracts of various cell lines using SFPQ Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using SFPQ Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat brain using SFPQ Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat brain using SFPQ Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using SFPQ Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using SFPQ Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse lung using SFPQ Polyclonal Antibody at dilution of  1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse lung using SFPQ Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SFPQ Polyclonal Antibody at dilution of  1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using SFPQ Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
SFPQ Polyclonal Antibody
Catalog Number
RD80169A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human SFPQ (NP_005057.1).
Gene ID
6421
Swissprot
P23246
Synonyms
SFPQPOMP100PPP1R140PSF
Calculated MW
72 kDa/76 kDa
Observed MW
115 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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