SHMT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80961A
Western blot analysis of extracts of various cell lines using SHMT1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat kidney using SHMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse kidney using SHMT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using SHMT1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
SHMT1 Polyclonal Antibody
Catalog Number
RD80961A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human SHMT1 (NP_683718.1).
Gene ID
6470
Swissprot
P34896
Synonyms
SHMT1CSHMTSHMT
Calculated MW
37 kDa/44 kDa/49 kDa/53 kDa
Observed MW
53 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas