SIM2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD82003A
Western blot analysis of extracts of various cell lines using SIM2 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using SIM2 Polyclonal Antibody at dilution of 1:1000.
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Overview

Circle
Product Name
SIM2 Polyclonal Antibody
Catalog Number
RD82003A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human SIM2 (NP_033664.2).
Gene ID
6493
Swissprot
Q14190
Synonyms
SIM2HMC13F06HMC29C01SIMbHLHe15
Calculated MW
63 kDa/73 kDa
Observed MW
56 kDa

Applications

Circle
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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