SIRT1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71779A
Western Blot analysis of K562 cells using SIRT1 Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Rat liver tissue using SIRT1 Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat lung tissue using SIRT1 Polyclonal Antibody at dilution of 1:200.
Product Name
SIRT1 Polyclonal Antibody
Catalog Number
RD71779A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins witHuman, Mouseono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Synthesized peptide derived from human SIRT1 around the non-phosphorylation site of Ser47.
Swissprot
Q96EB6
Synonyms
75SirT1hSIR2hSIRT1HST2S. cerevisiaehomolog ofNAD dependent deacetylase sirtuin 1NAD dependent protein deacetylase sirtuin 1OTTHUMP00000198111OTTHUMP00000198112Regulatory protein SIR2 homolog 1SIR1SIR2SIR2 like 1SIR2 like protein 1SIR2S.cer
Calculated MW
82 kDa
Observed MW
81 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:5000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.