SMAD7 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD80877A
Western blot analysis of extracts of various cell lines using SMAD7 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of U2OS cells using SMAD7 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
SMAD7 Polyclonal Antibody
Catalog Number
RD80877A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human SMAD7 (NP_005895.1).
Gene ID
4092
Swissprot
O15105
Synonyms
SMAD7CRCS3MADH7MADH8
Calculated MW
23 kDa/46 kDa
Observed MW
46 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas