Western blot analysis of extracts of various cell lines using Snail Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using Snail Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Immunogen Information
Immunogen
A synthetic peptide of human Snail
Gene ID
6615
Swissprot
O95863
Synonyms
SNAI1SLUGH2SNASNAHSNAILSNAIL1dJ710H13.1
Calculated MW
50kDa/51kDa
Observed MW
65kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.