SOD2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Immunohistochemistry of paraffin-embedded rat kidney using SOD2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon using SOD2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using SOD2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunofluorescence analysis of C6 cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90348A-IF01.jpg)
Immunofluorescence analysis of C6 cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90348A-IF02.jpg)
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
![Immunofluorescence analysis of U2OS cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](/upload/ec/antibodies/RD90348A-IF03.jpg)
Immunofluorescence analysis of U2OS cells using [KO Validated] SOD2 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Product Name
SOD2 Polyclonal Antibody
Catalog Number
RD90348A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Immunogen Information
Immunogen
Recombinant fusion protein of human SOD2
Gene ID
6648
Swissprot
P04179
Synonyms
SOD2IPO-BIPOBMNSODMVCD6Mn-SODsuperoxide dismutase 2
Calculated MW
75kDa/76kDa/82kDa/90kDa/95kDa
Observed MW
Refer to figures
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.