For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89540A
Western blot analysis of extracts of various cell lines using SOX1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded mouse embryos using SOX1 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.
Immunogen Information
Immunogen
A synthetic peptide of human SOX1
Gene ID
6656
Swissprot
O00570
Synonyms
SOX1SRY-box 1
Calculated MW
44kDa/46kDa/58kDa/61kDa
Observed MW
61KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.