SPARC Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD77596A
Western Blot analysis of A375 cell using SPARC Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human liver cancer using SPARC Polyclonal Antibody at dilution of 1:35
Product Name
SPARC Polyclonal Antibody
Catalog Number
RD77596A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.
Immunogen Information
Immunogen
Recombinant protein of human SPARC
Swissprot
P09486
Synonyms
AA517111Basement membrane protein 40Basement-membrane protein 40BM 40BM-40BM40Cysteine rich proteinhm:zeh0062MGC128090OI17ONOsteonectinSecreted acidic cystein rich glycoproteinSecreted protein acidic and cysteine richSecreted protein acidic
Calculated MW
35 kDa
Gene Accession
BC004974
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:25-1:100
Concentration
0.5 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.