SPARC Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD81807A
Western blot analysis of extracts of A375 cells using SPARC Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of C6 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SPARC Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
SPARC Polyclonal Antibody
Catalog Number
RD81807A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human SPARC (NP_003109.1).
Gene ID
6678
Swissprot
P09486
Synonyms
SPARCBM-40OI17
Calculated MW
34 kDa
Observed MW
35 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.