For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88331A
Western blot analysis of extracts of various cell lines using SPECC1L Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human esophageal using SPECC1L Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.
Immunogen Information
Immunogen
Recombinant fusion protein of human SPECC1L
Gene ID
23384
Swissprot
Q69YQ0
Synonyms
SPECC1LCYTSAGBBB2OBLFC1
Calculated MW
35kDa/44kDa
Observed MW
42kDa
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.