Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors,Jak kinases (TYK2 and JAK1) are activated,leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize,associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor,that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes,which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma),STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF),migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes,inducing a cellular antiviral state.
Immunogen Information
Immunogen
Recombinant Human STAT1 Protein expressed by E.coli
Swissprot
P42224
Synonyms
Signal transducer and activator of transcription 1 91kDCANDF7DKFZp686B04100IMD31AIMD31BIMD31CISGF 3ISGF-3OTTHUMP00000163552OTTHUMP00000165046OTTHUMP00000165047OTTHUMP00000205845Signal transducer and activator of transcription 1 91kDaSignal tr
Calculated MW
15kDa
Observed MW
15kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.