Strep-Tag II Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86711A
Western Blotting of Strep Ⅱ-Tag fused recombinant protein with Anti-Strep Ⅱ mouse monoclonal antibody at dilution of 1:1000.
Product Name
Strep-Tag II Monoclonal Antibody
Catalog Number
RD86711A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG1
Host
Mouse
Background
The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.
Immunogen Information
Immunogen
Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin.
Synonyms
Strep Tag IIStrep II
Calculated MW
32kDa
Observed MW
32kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000
Concentration
4.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.