Streptavidin Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86652A
Western blotting of Streptavidin with anti-Streptavidin rabbit polyclonal antibody at dilution of 1:1000. Lane 1 : Recombinant Streptavidin protein at 6.25ng; Lane 2 : Recombinant Streptavidin protein at 12.5ng; Lane 3: Recombinant Streptavidin protein at 25ng; Lane 4: Recombinant Streptavidin protein at 50ng
Product Name
Streptavidin Polyclonal Antibody
Catalog Number
RD86652A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces. Streptavidin can bind to four moles of biotin per mole of protein with extremely high affinity, approximately 10,000-15,000 Da. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point of 6.5 to avidin's 10 far closer to that at which most useful biological interactions occur. As a result, Streptavidin frequently exhibits much lower non-specific binding than avidin does.
Immunogen Information
Immunogen
Native Streptomyces Avidinii streptavidin protein
Swissprot
P22629
Synonyms
StreptavidinSAAvidin
Calculated MW
26kDa
Observed MW
26kDa
Applications
Reactivity
All
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
2.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.