SUPT16H Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89160A
Western blot analysis of extracts of various cell lines using SUPT16H Polyclonal Antibody at1:1000 dilution.
Western blot analysis of extracts of Mouse brain using SUPT16H Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of U2OS using SUPT16H Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 using SUPT16H Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
SUPT16H Polyclonal Antibody
Catalog Number
RD89160A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.
Immunogen Information
Immunogen
Recombinant fusion protein of human SUPT16H
Gene ID
11198
Swissprot
Q9Y5B9
Synonyms
CDC68SPT16FACTP140SPT16/CDC68
Calculated MW
31kDa/37kDa
Observed MW
25KDa/37KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.