Tenascin C Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89020A
Western blot analysis of U-138MG using Tenascin C Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using Tenascin C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U-87MG cells using Tenascin C Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Product Name
Tenascin C Polyclonal Antibody
Catalog Number
RD89020A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]
Immunogen Information
Immunogen
Recombinant fusion protein of human Tenascin C
Gene ID
3371
Swissprot
P24821
Synonyms
150-225DFNA56GMEMGPHXBJITNTN-CTNC
Calculated MW
25kDa
Observed MW
27KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas