TET2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90262A
Western blot analysis of extracts of F9 cells using TET2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat brain using TET2 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of F9 cells using TET2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
TET2 Polyclonal Antibody
Catalog Number
RD90262A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC into 5-hydroxymethylcytosine (5hmC and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC, and conversion of 5fC to 5-carboxylcytosine (5caC. Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.
Immunogen Information
Immunogen
Recombinant fusion protein of mouse TET2
Gene ID
214133
Swissprot
Q4JK59
Synonyms
Ayu17-44Ayu17-449mKIAA1546E130014J05Rik
Calculated MW
194kDa
Observed MW
230KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.