TFR2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using TFR2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using TFR2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using TFR2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
TFR2 Polyclonal Antibody
Catalog Number
RD90022A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a single-pass type II membrane protein, which is a member of the transferrin receptor-like family. This protein mediates cellular uptake of transferrin-bound iron, and may be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human TFR2
Gene ID
7036
Swissprot
Q9UP52
Synonyms
TFR2HFE3TFRC2
Calculated MW
84kDa/89kDa
Observed MW
84kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.