TIGAR Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89275A
Western blot analysis of extracts of various cell lines using TIGAR Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using TIGAR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using TIGAR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using TIGAR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
TIGAR Polyclonal Antibody
Catalog Number
RD89275A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region.
Immunogen Information
Immunogen
A synthetic peptide of human TIGAR
Gene ID
57103
Swissprot
Q9NQ88
Synonyms
FR2BPC12orf5
Calculated MW
37kDa
Observed MW
45KDa/46KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas