TNF alpha Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD71868A
Western Blot analysis of Jurkat cells with TNF alpha Polyclonal Antibody
Immunofluorescence analysis of Human stomach tissue using TNF alpha Polyclonal Antibody at dilution of 1:200.
Product Name
TNF alpha Polyclonal Antibody
Catalog Number
RD71868A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Immunogen Information
Immunogen
Synthesized peptide derived from the Internal region of human TNF-α
Swissprot
P01375
Synonyms
APC1APC1 proteinCachectinDIFDifferentiation inducing factorMacrophage cytotoxic factorTnfTNF superfamilymember 2TNFmacrophage derivedTNFmonocyte derivedTNF-aTNF-alphaTNFATNFATNFSF2Tumor necrosis factor alphaTumor necrosis factorTumor n
Calculated MW
26 kDa
Observed MW
16 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC-p,IF,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas