TNF-α Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Immunohistochemistry of paraffin-embedded human colon carcinoma using TNF-α Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using TNF-α Polyclonal Antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat spleen using TNF-α Polyclonal antibody at dilution of 1:25 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Human mammary cancer cells using TNF-α Polyclonal antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
TNF-α Polyclonal Antibody
Catalog Number
RD86737A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Immunogen Information
Immunogen
Recombinant fusion protein of human TNF
Gene ID
7124
Swissprot
P01375
Synonyms
TNFDIFTNF-alphaTNFATNFSF2TNLG1FTNF alpha
Calculated MW
564kDa/565kDa
Observed MW
564KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas