TNNI3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD84206A
Western blot analysis of extracts of various cell lines using TNNI3 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of Mouse heart cells using TNNI3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
TNNI3 Polyclonal Antibody
Catalog Number
RD84206A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
Immunogen Information
Immunogen
Recombinant fusion protein of human TNNI3 (NP_000354.4).
Gene ID
7137
Swissprot
P19429
Synonyms
TNNI3CMD1FFCMD2ACMH7RCM1TNNC1cTnItroponin I3
Calculated MW
24 kDa
Observed MW
26 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.