TRAF7 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of extracts of various cell lines using TRAF7 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TRAF7 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using TRAF7 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TRAF7 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using TRAF7 Polyclonal Antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Product Name
TRAF7 Polyclonal Antibody
Catalog Number
RD89542A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.
Immunogen Information
Immunogen
Recombinant fusion protein of human TRAF7
Gene ID
84231
Swissprot
Q6Q0C0
Synonyms
TRAF7RFWD1RNF119
Calculated MW
38kDa/41kDa
Observed MW
39KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000,IHC 1:50-1:100,IF 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas