TRIM5 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD82385A
Western blot analysis of extracts of various cell lines using TRIM5 Polyclonal Antibody at dilution of 1:1000.
Product Name
TRIM5 Polyclonal Antibody
Catalog Number
RD82385A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human TRIM5 (NP_149083.2).
Gene ID
85363
Swissprot
Q9C035
Synonyms
TRIM5RNF88TRIM5alpha
Calculated MW
29 kDa/31 kDa/37 kDa/40 kDa/46 kDa/56 kDa
Observed MW
50 kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.