TRMT112 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD87843A
Western blot analysis of extracts of various cell lines using TRMT112 Polyclonal Antibody at 1:3000 dilution.
Western blot analysis of extracts of various cell lines using TRMT112 Polyclonal Antibody at 1:3000 dilution.
Variation
Quantity
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Overview
Circle
Product Name
TRMT112 Polyclonal Antibody
Catalog Number
RD87843A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Circle
Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase BUD23, methylates the N(7 position of a guanine in 18S rRNA. The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with HEMK2/N6AMT1 also monomethylates 'Lys-12' of histone H4 (H4K12me1. The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production. Together with methyltransferase METTL5, specifically methylates the 6th position of adenine in position 1832 of 18S rRNA.
Immunogen Information
Circle
Immunogen
Recombinant fusion protein of human TRMT112
Gene ID
51504
Swissprot
Q9UI30
Synonyms
TRMT112HSPC152HSPC170TRM112TRMT11-2hTrm112
Calculated MW
64kDa/66kDa
Observed MW
75kDa
Applications
Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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