For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89399A
Western blot analysis of extracts of various cell lines using TRMT2A/HTF9C Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human lung cancer using TRMT2A/HTF9C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human TRMT2A/HTF9C
Gene ID
27037
Swissprot
Q8IZ69
Synonyms
TRMT2AHTF9CtRNA methyltransferase 2 homolog A
Calculated MW
23kDa
Observed MW
Refer to figures
Applications
Reactivity
Human
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.