For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89860A
Western blot analysis of extracts of various cell lines using TROP-2 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Mouse kidney using TROP-2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TROP-2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A431 cells using TROP-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.
Immunogen Information
Immunogen
Recombinant fusion protein of human TROP-2
Gene ID
4070
Swissprot
P09758
Synonyms
TACSTD2EGP-1EGP1GA733-1GA7331GP50M1S1TROP2
Calculated MW
42kDa
Observed MW
42kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.