For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88479A
Western blot analysis of extracts of various cell lines using TRPV6 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat stomach using TRPV6 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
This gene encodes a member of a family of multipass membrane proteins that functions as calcium channels. The encoded protein contains N-terminal ankyrin repeats, which are required for channel assembly and regulation. Translation initiation for this protein occurs at a non-AUG start codon that is decoded as methionine. This gene is situated next to a closely related gene for transient receptor potential cation channel subfamily V member 5 (TRPV5). This locus has experienced positive selection in non-African populations, resulting in several non-synonymous codon differences among individuals of different genetic backgrounds.
Immunogen Information
Immunogen
A synthetic peptide of human TRPV6
Gene ID
55503
Swissprot
Q9H1D0
Synonyms
TRPV6ABP/ZFCAT1CATLECAC2HSA277909LP6728ZFAB
Calculated MW
207kDa/212kDa/215kDa/216kDa
Observed MW
220kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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