Western blot analysis of extracts of various cell lines using TRRAP Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TRRAP Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Immunogen Information
Immunogen
Recombinant fusion protein of human TRRAP
Gene ID
8295
Swissprot
Q9Y4A5
Synonyms
PAF350/400PAF400STAF40TR-APTra1
Observed MW
320KDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.