For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87110A
Western blot analysis of extracts of various cell lines using TSC2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using TSC2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human thyroid cancer using TSC2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using TSC2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Information
Immunogen
Recombinant fusion protein of human TSC2
Gene ID
7249
Swissprot
P49815
Synonyms
LAMPPP1R160TSC4TSC2Tuberintuberin
Calculated MW
60kDa/63kDa/64kDa/67kDa
Observed MW
62kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:200-1:500,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.