For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86670A
Western blotting with anti-Tyrosine hydroxylase polyclonal antibody at dilution of 1:1000. Lane 1: Mouse brain lysate, Lane 2: PC-12 cell lysate, lane 3: Rat brain lysate.
TH (Tyrosine 3-hydroxylase), also known as Tyrosine 3-monooxygenase, is a 60-62 kDa member of the biopterin-dependent aromatic amino acid hydroxylase family of molecules. It is expressed by neurons of the dopamine and autonomic nervous system, plus the neuroendocrine cells of the adrenal medulla. TH is considered the rate limiting enzyme for catecholamine synthesis, and serves to catalyze the hydroxylation of L-tyrosine.
Immunogen Information
Immunogen
Recombinant Mouse Tyrosine hydroxylase protein expressed by E.coli.
Gene ID
21823
Swissprot
P24529
Synonyms
ThTY3HTHP
Calculated MW
34kDa
Observed MW
40kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
2.5mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.