UBC Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD82878A
Western blot analysis of extracts of various cell lines using UBC Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat liver using UBC Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophageal cancer using UBC Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse brain using UBC Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using UBC Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
UBC Polyclonal Antibody
Catalog Number
RD82878A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways.
Immunogen Information
Immunogen
A synthetic peptide of human UBC (NP_066289.3).
Gene ID
7316
Swissprot
P0CG48
Synonyms
UBCHMG20ubiquitin C
Calculated MW
77 kDa
Observed MW
12-55 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:1000-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.